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qiagen 59104 甲基化轉(zhuǎn)化試劑盒EpiTect Bisulfite Kit

  • 更新時間:  2023-07-26
  • 產(chǎn)品型號:  
  • 簡單描述
  • qiagen 59104 甲基化轉(zhuǎn)化試劑盒EpiTect Bisulfite Kit
    亞硫酸氫鹽的*轉(zhuǎn)化和DNA回收,用于甲基化分析
    簡化的流程,獲得快速、可靠的結(jié)果
    胞嘧啶*轉(zhuǎn)化
    *的DNA保護體系,用于高度敏感的分析
    優(yōu)化的試驗方案可處理FFPE組織樣本DNA
    離心柱和高通量96孔板方式
詳細介紹

qiagen 59104 甲基化轉(zhuǎn)化試劑盒EpiTect Bisulfite Kit

亞硫酸氫鹽的*轉(zhuǎn)化和DNA回收,用于甲基化分析

  • 簡化的流程,獲得快速、可靠的結(jié)果
  • 胞嘧啶*轉(zhuǎn)化
  • *的DNA保護體系,用于高度敏感的分析
  • 優(yōu)化的試驗方案可處理FFPE組織樣本DNA
  • 離心柱和高通量96孔板方式
  • EpiTect Bisulfite Kit可在6小時內(nèi)將DNA 序列中非甲基化的胞嘧啶*轉(zhuǎn)化為尿嘧啶。EpiTect 96 Bisulfite操作時間少于7小時。高靈敏度的方法采用全新的保護機制防止DNA降解,確保即使是1 ng的DNA也可獲得靈敏的結(jié)果,轉(zhuǎn)化率可高達99%。得益于*的DNA保護技術(shù),EpiTect Bisulfite轉(zhuǎn)化后DNA高度適用于EpiTect Whole Bisulfitome Kits轉(zhuǎn)化后全基因組擴增。因此可在轉(zhuǎn)化后DNA量較少的情況下確保可靠的擴增。EpiTect Bisulfite Kits包括離心柱或96孔板形式的擴增和脫磺酸基過程,確保快速可靠的實驗結(jié)果用于下游應(yīng)用。兩個試劑盒都可用于離心機或真空裝置,另外,EpiTect Bisulfite Kit可在QIAcube全自動核酸純化儀上全自動運行。

qiagen 59104 甲基化轉(zhuǎn)化試劑盒EpiTect Bisulfite Kit

Performance

快速得到實驗結(jié)果

完整的EpiTect離心和真空過程只需6小時,包括從DNA到待分析的亞硫酸氫鹽轉(zhuǎn)化后的高純DNA。EpiTect 96 Bisulfite操作時間少于7小時。傳統(tǒng)的亞硫酸氫鹽轉(zhuǎn)化過程需要18個小時,且需要大量的操作(參見"快速簡單的亞硫酸氫鹽轉(zhuǎn)化"和"Time saving procedure")。EpiTect Bisulfite Kit為成功進行亞硫酸氫鹽轉(zhuǎn)化和DNA回收提供所需的全部試劑。

快速簡單的亞硫酸氫鹽轉(zhuǎn)化
 EpiTect流程傳統(tǒng)流程
反應(yīng)構(gòu)建10分鐘;預制溶液40分鐘;需反應(yīng)構(gòu)建
亞硫酸氫鹽轉(zhuǎn)化5小時16小時
純化30–45分鐘; EpiTect離心柱或孔板120分鐘;純化、脫璜、沉淀和洗滌

訂購信息:

EpiTect Bisulfite Kit (48)280059104
EpiTect 96 Bisulfite Kit (2)999059110
EpiTect Plus DNA Bisulfite Kit (48)225059124
EpiTect Whole Bisulfitome Kit (25)456059203
EpiTect MSP Kit (100)360059305
EpiTect MethyLight PCR Kit (200)1034059436
EpiTect HRM PCR Kit (100)144059445
EpiTect MethyLight PCR +ROX Kit (200)1060059496
EpiTect Control DNA (1000)273059568
EpiTect Control DNA, methylated (100)273059655
EpiTect Control DNA, unmethylated (100)273059665
EpiTect PCR Control DNA Set (100)546059695
EpiTect Fast 96 DNA Bisulfite Kit1091059720
EpiTect Fast 96 FFPE Bisulfite Kit1931059740
EpiTect Fast Bisulfite Kit (10)84759802
EpiTect Fast DNA Bisulfite Kit (50)243059824
EpiTect Fast DNA Bisulfite Kit (200)829059826
EpiTect Fast FFPE Bisulfite Kit (50)424059844
EpiTect Fast LyseAll Bisulfite Kit (50)272059864
EpiTect Fast LyseAll Bisulfite Kit (200)927059866
QIAamp DSP Virus Kit (50),CE452060704
QIAamp DSP DNA Blood Mini Kit (50), CE253061104
QIAamp DSP Virus Spin Kit (50)358061704
MagAttract HMW DNA Kit (48)397067563
DNeasy Plant Maxi Kit (24)459068163
DNeasy Plant Mini Kit (50)275069104
DNeasy Plant Mini Kit (250)1231069106
DNeasy 96 Plant Kit (6)1159069181
DNeasy Blood & Tissue Kit (50)214069504
DNeasy Blood & Tissue Kit (250)881069506
DNeasy mericon Food Kit (50)199069514
DNeasy mericon 96 QIAcube HT Kit (5)1143069571
DNeasy 96 Blood & Tissue Kit (4)878069581
DNeasy 96 Blood & Tissue Kit (12)2424069582
TissueLyser Single-Bead Dispenser, 5mm352069965
TissueLyser Single-Bead Dispenser, 7mm352069967
TissueLyser 3 mm Bead Dispenser, 96-well1016069973
TissueLyser 5 mm Bead Dispenser, 96-well1016069975
TissueLyser LT Adapter, 12-Tube1725069980
TissueLyser Adapter Set (2 x 24)1627069982
TissueLyser Adapter Set (2 x 96)1220069984
RNeasy Protect Animal Blood Kit (50)658073224
Rneasy Microarray Tissue Mini Kit (50)535073304
RNeasy Plus Universal Tissue Mini Kit50533073404
RNeasy Plus Universal Tissue Midi Kit10282073442
RNeasy FFPE Kit (50)581073504
RNeasy Micro Kit (50)660074004
RNeasy Plus Micro Kit (50)572074034
RNeasy Mini Kit (50)416074104
RNeasy Mini Kit (250)1752074106
RNeasy Mini QIAcube Kit (240)1928074116
RNeasy Protect Mini Kit (50)500074124
RNeasy Protect Mini Kit (250)2195074126
RNeasy Plus Mini Kit (50)476074134
RNeasy Plus Mini Kit (250)2006074136
RNeasy 96 QIAcube HT Kit (5)1487074171
RNeasy 96 Kit (4)2109074181
RNeasy 96 Kit (12)5797074182
Rneasy Plus 96 Kit (12)6681074192
RNeasy MinElute Cleanup Kit (50)472074204
RNeasy Protect Saliva Mini Kit (50)992074324
RNeasy FFPE Kit (50)594074404
RNeasy Protect Bacteria Mini Kit (50)657074524
RNeasy Protect Cell Mini Kit (50)500074624
RNeasy Fibrous Tissue Mini Kit (50)542074704
RNeasy Lipid Tissue Mini Kit (50)543074804
RNeasy 96 Universal Tissue Kit (4)2628074881
RNeasy Plant Mini Kit (20)209074903
RNeasy Plant Mini Kit (50)486074904
RNeasy Midi Kit (10)207075142
RNeasy Midi Kit (50)923075144
RNeasy Maxi Kit (12)490075162
exoEasy Maxi Kit (20)672076064
RNAlater (50ml)114076104

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